The COVID-19 Pandemic and the State of Emergency: Lessons from Portugal

The present chapter analyses the normative response of Portugal to the coronavirus crisis. As in other European Member States, the reaction to the pandemic was developed through a combination of enactment of the constitutional state of emergency with the statutory administrative emergency regime. Under both frameworks, there has been a strengthening of the executive at the expense of a parliamentary retreat. This chapter covers both responses to the pandemic and their shortcomings, mainly regarding the lack of democratic legitimation of the fight against the pandemic in face of a parliamentary circumvention and abdication. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022.

Separation and Discrimination: The Lived Experience of COVID-19 Survivors in Philippine Isolation Centers

Social isolation in times of pandemic can affect the well-being of individuals infected with a contagious disease. This study explores the lived experience of the 12 COVID-19 survivors placed in community-based isolation centers in Cebu City, Philippines and whose cases were mild and asymptomatic. In describing their lived experience, we employed Max van Manen's phenomenology of practice. Results show that the COVID-19 survivors have suffered more from the consequences of separation and discrimination than the disease's physiological effects. Educating the whole community about social responsibility and ethical behavior in dealing with COVID-19 survivors is essential to minimize social stigma and discrimination.

Social disability and impairment in childhood anxiety

This chapter discusses social disability and impairment in childhood anxiety. The chapter starts with a discussion of clinical characteristics of the condition. Approaches to evidence-based assessment and cognitive-behavioral treatment are discussed. Future directions are highlighted. © Springer Nature Switzerland AG 2011, 2023. All rights reserved.

Special Issue: Cytometry and analytical methods in Covid-SARS-2 diagnostics and potential treatment. (Special Issue: Cytometry and analytical methods in Covid-SARS-2 diagnostics and potential treatment.)

This special issue contains 9 articles that discuss quantitative single-cell analysis, features original research reports and reviews of innovative scientific studies employing quantitative single-cell measurement, separation, manipulation, and modeling techniques, as well as original articles on mechanisms of molecular and cellular functions obtained by cytometry technique, particularly focusing on several research articles and reviews on exploring the use of flow cytometry techniques towards clinical opportunities in the detection and diagnostics of COVID-19.

The interplay between viral molecular mimicry and host chromatin dynamics.

Molecular mimicry is a commonly used mechanism by viruses to manipulate host cellular machinery and coordinate their life cycles. While histone mimicry is well studied, viruses also employ other mimicry strategies to affect chromatin dynamics. However, the relationship between viral molecular mimicry and host chromatin regulation is not well understood. This review summarizes recent advances in histone mimicry and explores how viral molecular mimicry influences chromatin dynamics. We also discuss how viral proteins interact with both intact and partially unfolded nucleosomes and compare the distinct mechanisms governing chromatin tethering. Finally, we address the role of viral molecular mimicry in regulating chromatin dynamics. This review provides new insights into viral molecular mimicry and its impact on host chromatin dynamics, paving the way for the development of novel antiviral strategies.

Knowledge Transfer in Support of the Development of Oxygen Concentrators in Emergency Settings During the COVID-19 Pandemic

The COVID-19 pandemic simultaneously disrupted supply chains and generated an urgent demand in medical infrastructure. Among personal protective equipment and ventilators, there was also an urgent demand for chemical oxygen. As devices to purify oxygen could not be manufactured and shipped rapidly enough, a simple and accessible oxygen concentrator based on pressure swing adsorption was developed at ETH Zurich in spring 2020. Instead of building devices locally and shipping them, it was decided to educate others in need of oxygen. The implementation encompassed education on process chemistry, material choice, and assembly and optimization of the concentrator and was realized using synchronous teaching tools, such as video call, and asynchronous ones, such as a website and video streaming. The project gained traction and interaction with engineering teams from universities and non-Governmental Organizations (Red Cross and the UN Development Program) in developing countries and emerging market economies, including Ecuador, Mexico, Somalia, and Peru. At the end of the project, the teams were surveyed regarding their experience in the educative knowledge transfer. It was reported that the learning experience prepared these groups well to build the device and to teach others as well. Major challenges were accessing some parts of the device and optimizing its performance. While synchronous communication is expected to be a very effective teaching method, the survey results showed that explanations via a website and video streaming have contributed the most to the implementation of the oxygen concentrator and thereby provide autonomous and sustainable education tools.

Revisiting the policy implications of COVID-19, asylum seekers, and migrants on the Mexico–U.S. border: Creating (and maintaining) states of exception in the Trump and Biden administrations

Public policy choices continue to bring dramatic changes to migration practices in the era of the coronavirus in the United States. In this article, we argue that the COVID-19 pandemic facilitated the creation and maintenance of states of exception while continuing to destabilize practices at the Mexico–U.S. border through the politics of fear. Specifically, the Migrant Protection Protocols (MPP), Zero Tolerance Policy (ZTP), COVID-19 CAPIO, Asylum Cooperative Agreements (ACA), and Title 42 used an arcane section of U.S. law to immediately expel asylum seekers and refugees. We show that these policies highlight the formation and maintenance of states of exception consistent with the work of Agamben. We further discuss how the politics of fear can reinforce hegemonic narratives targeting asylum seekers while shaping political agendas that lean toward a specific brand of nationalism using public health as a context. The U.S. government under the Trump administration—and the Biden administration to a lesser, yet continuous, extent—constructed these policies aimed primarily at refugees and asylum seekers from El Salvador, Guatemala, Honduras, and Mexico thereby violating laws and international treaty obligations. Related Articles: Duman, Yoav H. 2014. "Reducing the Fog? Immigrant Regularization and the State.” Politics & Policy 42(2): 187–220. https://doi.org/10.1111/polp.12065. Garrett, Terence Michael. 2020. "The Security Apparatus, Federal Magistrate Courts, and Detention Centers as Simulacra: The Effects of Trump's Zero Tolerance Policy on Migrants and Refugees in the Rio Grande Valley.” Politics & Policy 48(2): 372–95. https://doi.org/10.1111/polp.12348. Maggio, James. 2007. "The Presidential Rhetoric of Terror: The (Re)Creation of Reality Immediately after 9/11.” Politics & Policy 35(4): 819–35. https://doi.org/10.1111/j.1747-1346.2007.00085.x. © 2023 Policy Studies Organization.

Kept apart: Routine family separation in the UK family immigration system as times of crises

The UK's family immigration regime involves the routine separation of partners from their families. Most obviously, it keeps apart those who are unable to meet the income and other requirements for family (re)unification, and those refused visas. But separation for at least several months, and sometimes much longer, is the norm even for those whose applications are eventually successful. This article draws on creative, co-produced accounts of immigration-related separation to reveal multi-faceted temporalities of crisis in the 'experiential migrantisation' of British citizens seeking to reunite bi-national families in the UK. The bureaucratic temporalities of immigration control impede aspirations for life-course progression and shared futures, while increasing the tempo of working and caring lives. In exploring the accounts of British citizens kept apart from partners by the immigration regime through a temporal lens, we chart this experiential migrantisation through the varied and intersecting temporalities of bureaucracy and immigration control, and of biography and (transnational) family life. These can become intertwined with and compound other temporalities of crisis at different levels, from the global Covid-19 pandemic and other international geo-political events, to the more intimate and familial, leading to 'times of crises'. Such crises are, moreover, often expressed through temporal tropes of key dates missed-birthdays, anniversaries, holidays-and phases of family life postponed.

Separation, Loss, Confinement, and Change: How Evagrius Can Speak to the Experience of Lockdown

Separation, loss, confinement, and change have been imposed on entire populations du-ring the Covid-19 pandemic in the form of lockdowns aimed at limiting the spread of the virus. They are also central to Evagrian asceticism, where they establish the conditions for the change at which the monastic life aims, namely to begin to reverse the effects of the fall by restoring the soul to health. This paper examines how they do so in order to gain an understanding of their ascetic function. Following the Introduction, it outlines Evagrius' anthropology in order to lay the groundwork for its main theme, the healing of pathos through the practical life. Separation, loss, and confinement are each considered in turn by referencing Evagrius' descriptions of their ascetic function and his own experience of them. Next, the change at which they aim is described, again drawing upon a range of Evagrian material. The final section of the paper considers how Evagrius can speak to the experience of lockdown by endowing separation, loss, confinement with meaning and purpose in relation to spiritual awakening and growth, highlighting our freedom to choose our attitude to them, and acting as both our guide and our companion. © 2022, John Paul II Catholic University of Lublin. All rights reserved.

Unearthing mask waste separation behavior in COVID-19 pandemic period: An empirical evidence from Ghana using an integrated theory of planned behavior and norm activation model.

The outbreak of the COVID-19 pandemic has precipitated a surge in mask usage around the world. This situation could spur environmental consequences which when not well managed, may compound waste and waste management, and further drive the spread of viral infections. Therefore, this study explores the antecedents of mask waste separation behavior in Ghana using an integrated model composed of the theory of planned behavior and the norm activation model. With the use of the questionnaire survey approach, data was drawn from 570 respondents, and further analyzed using the structural equation modeling technique. Results revealed that the relationship between awareness of consequences and other constructs, except attitude, and the relationship between ascription of responsibility and other constructs, except attitude, are significant. Moreover, significant relationships between subjective norm and attitude, subjective norm and personal norm, and subjective norm and perceived behavior control were validated. However, no significant relationship exists between awareness of consequences and attitude, and between awareness of responsibility and attitude. On the basis of the study findings, insightful implications for policy and suggestions for future research are presented.

Modular characterization of SARS-CoV-2 nucleocapsid protein domain functions in nucleocapsid-like assembly.

SARS-CoV-2 and its variants, with the Omicron subvariant XBB currently prevailing the global infections, continue to pose threats on public health worldwide. This non-segmented positive-stranded RNA virus encodes the multi-functional nucleocapsid protein (N) that plays key roles in viral infection, replication, genome packaging and budding. N protein consists of two structural domains, NTD and CTD, and three intrinsically disordered regions (IDRs) including the NIDR, the serine/arginine rich motif (SRIDR), and the CIDR. Previous studies revealed functions of N protein in RNA binding, oligomerization, and liquid-liquid phase separation (LLPS), however, characterizations of individual domains and their dissected contributions to N protein functions remain incomplete. In particular, little is known about N protein assembly that may play essential roles in viral replication and genome packing. Here, we present a modular approach to dissect functional roles of individual domains in SARS-CoV-2 N protein that reveals inhibitory or augmented modulations of protein assembly and LLPS in the presence of viral RNAs. Intriguingly, full-length N protein (NFL) assembles into ring-like architecture whereas the truncated SRIDR-CTD-CIDR (N182-419) promotes filamentous assembly. Moreover, LLPS droplets of NFL and N182-419 are significantly enlarged in the presence of viral RNAs, and we observed filamentous structures in the N182-419 droplets using correlative light and electron microscopy (CLEM), suggesting that the formation of LLPS droplets may promote higher-order assembly of N protein for transcription, replication and packaging. Together this study expands our understanding of the multiple functions of N protein in SARS-CoV-2.

Modifications in SARS-CoV-2 N Linker Region Regulate RNA Interactions and Phase Separation

The N protein of the SARS-CoV-2 virion is critical for viral genome packaging via RNA binding and regulation of viral transcription at the replication-transcription complex (RTC). The N protein can be divided into five main domains, and the central region is the linker, which is predicted to be primarily disordered and has not been heavily studied. The linker is Serine-Arginine Rich, which is phosphorylated at multiple sites by host kinases during infection, thereby promoting the N protein's role in viral transcription. Phosphorylation is a critical process for the regulation of many cellular processes and can provide recognition sites for binding complexes. In a study that examined the recognition of the SARS-CoV-2 N protein by the human 14-3-3 protein, the linker was found to contain critical phosphosites for 14-3-3 binding. The goals of this project are to determine the structure, dynamics, and RNA interactions of the Serine-Arginine Rich linker region. To accomplish this, we performed Nuclear Magnetic Resonance spectroscopy (NMR) experiments to analyze the structure of the linker region of the N protein and its ability to bind viral RNA. NMR confirms predictions that the linker is not entirely unstructured and it is able to bind RNA. The linker region of the N protein with phosphoserine incorporated at S188 was also examined via an NMR titration experiment with 1-1000 RNA. Compared to wild type, the incorporation of phosphorylation decreases binding. Other biophysical techniques such as Analytical Ultracentrifugation (AUC) and Multi-Angle Light Scattering (MALS) are used to identify the association state of the linker and the size of the resulting protein-RNA complex. We are currently working to biophysically characterize the structure, dynamics, and viral RNA binding ability of a mutation found in the Delta and Omicron variants: the R203M linker, which have been shown to enhance viral infectivity. This work was supported by the NSF EAGER grant NSF/ MCB 2034446 and URSA Engage. Support to facilities includes the Oregon State University NMR Facility funded in part by NIH, HEI Grant 1S10OD018518, and by the M. J. Murdock Charitable Trust grant # 2014162.Copyright © 2023 The American Society for Biochemistry and Molecular Biology, Inc.

Immigration Law's Boundary Problem: Determining the Scope of Executive Discretion

In immigration law, executive discretion has become contested terrain. Courts, officials, and scholars have rarely distinguished between regulatory discretion, which facilitates exclusion and removal of noncitizens, and protective discretion, which safeguards noncitizens' reliance interests. Moreover, courts have long discerned an internal-external divide in discretion, deferring to executive measures that exclude noncitizens abroad, while reducing deference for measures concerning noncitizens who have already entered the United States. Immigration law needs a cohesive framework for executive discretion. This Article suggests a stewardship model to fill that gap.Recent developments have emphasized the need for a coherent model of discretion. The Trump Administration altered the landscape of executive discretion, seizing every chance to make the law harsher. The Biden Administration's efforts to correct this imbalance have been only partially successful. For example, the Biden Administration has issued a final rule supporting the Deferred Action for Childhood Arrivals (DACA) program and has issued enforcement guidelines that prioritize threats to national security and public safety and address recent irregular entries at the border. The Biden Administration has also sought to end the Trump Administration's "Remain in Mexico " program, which subjects tens of thousands of asylum seekers to peril. However, for over a year, President Biden retained the Title 42 program, which precluded asylum in the name of preventing the introduction of COVID-19. That program undercuts asylum and does not perform its ostensible public health mission. Only an unfavorable court decision in 2022 spurred efforts to terminate Title 42. At that point, another court enjoined Title 42's termination, illustrating yet again the confused state of executive discretion.A workable approach to executive discretion requires returning to first principles. To achieve these goals, the stewardship model highlights three factors: fit with the statutory framework, protection of reliance interests, and avoidance of adverse impacts on foreign relations. This Article applies these values to DACA, the Biden enforcement guidelines, Title 42, and the Remain in Mexico program.

Psychic separation: Adolescence, pandemic and psychoanalysis. Systematic review of the literature in Latin America

The present investigation was conducted in the pandemic period of covid-19. Given this panorama, it was necessary to know the current state of the research regarding the difficulties of adolescents related to the distancing of family subjectivity or "psychic separation" generated by confinement. Its main objective was to describe the psychoanalytic constructs that could explain the difficulty of psychic separation in adolescents in the face of the so-called New Normality. Thus, a systematic review of the literature was conducted from October 2020 to July 2021. The sample was made up of 12 documents from the Scielo database and the Google Scholar metasearch engine that provided research from university repositories. One of the most relevant results of the studies analyzed was the particular response to a frequent problem: The family group was decisive in building an "external" bridge, either by way of respecting the privacy of the adolescent or contributing to the insertion in other extra-family spaces. The bibliometric data showed that the greatest scientific production occurred in 2020, prioritizing articles over theses;on the other hand, Ecuador and Argentina were the ones that published the most.

Anxiety disorders and sleep

Anxiety disorders are the most common psychiatric illnesses in the world. As defined by the DSM-5, the key manual for the diagnosis of mental illnesses in the United States, only two anxiety disorders have sleep-related problems as part of their diagnostic criteria: generalized anxiety disorder (GAD) and separation anxiety disorder. GAD and panic disorder are the two most studied anxiety disorders with regard to sleep problems. This article reviews the demographics of anxiety disorders and sleep problems and also touches on alternative cultural idioms of anxiety that include sleep disruptions. The article also reviews sleep findings for GAD and panic disorder and discusses neurobiological evidence and some proposed mechanisms of the relationship between anxiety and sleep. Neurotransmitters and genetics involved are highlighted. Treatment, including psychotherapy and standard pharmacotherapy are emphasized, as is the evidence for alternative therapies such as herbal remedies. The article also makes note of recent studies on the interplay of anxiety, sleep, and COVID-19. © 2023 Elsevier Inc. All rights reserved

Lockdown time, time loops, and the crisis of the future

Amidst the Covid-19 lockdowns of 2020 and 2021 in the United States and United Kingdom, a fantasy took hold that life under lockdown was like living in a time loop. The time loop quickly became the genre of the moment. And yet, however "timely" they appeared, most of the time-loop films and series du jour had been conceived and produced before the pandemic. Why and how did they become retrofitted to the temporality of the pandemic? To answer this question, we delve into the split time of the time-loop film. We argue that, in its deferred arrival, the time loop became a fantastical solution to the problems of loneliness, stuckness, and the future that the pandemic stoked but did not originate. (PsycInfo Database Record (c) 2023 APA, all rights reserved)

EXTRACORPOREAL THERAPY IN CRITICALLY ILL PATIENTS WITH COVID-19: A SINGLE-CENTER EXPERIENCE.

Aim To assess the safety and efficacy of extracorporeal therapy in patients with COVID-19. The study included 27 patients aged 67+/-9.7 [min 38, max 87] years with a laboratory-confirmed SARS-CoV-2 and bilateral polysegmental pneumonia, various concomitant chronic diseases who were admitted to Intensive Care Unit and received extracorporeal therapies. All patients had the mean NEWS score of 6.9+/-2.7 [min 4, max 9] and the mean SOFA score of 8.1+/-3.1 [min 3, max 16] at admission Methods to the ICU. 19 patients (70.4%) had severe lung lesions over 75% according to the chest CT scans. 48 extracorporeal therapies were performed using the Multifiltrate (Fresenius Medical Care, Germany) and Aquarius (Nikkiso Aquarius RCA, Great Britain) medical devices. Indications for extracorporeal therapy initiation included cytokine storm associated with acute respiratory distress syndrome and septic shock. Generally, each patient received at least one extracorporeal therapy. 11 patients underwent 2 to 6 sessions. Isolated plasma separation and hemoperfusion helped to reduce vasopressor / cardiotonic support, slightly improved ventilation parameters, with a significant, but not long-term decrease in the levels of inflammation markers. Combining different modalities of extracorporeal therapy that provide rapid elimination of agents, controlled temperature response and hydration, maintaining Results homeostasis and detoxification, appeared to be most optimal. Extracorporeal therapy did not improve the volume of lung parenchyma or lung parenchyma damage. However, 19 (70.4%) patients who received extracorporeal therapy transitioned from mechanical ventilation to spontaneous breathing, whereas 8 (29.6%) patients had severe lung lesions (over 75%) according to the repeated chest CT scans. The mean length of stay in the ICU among survivors was 9+/-3.5 [min 4, max 22]. The 28-day mortality and in-hospital mortality rate was 25.9% (7). Prolonged extracorporeal therapy in patients with SARS-Cov-2 has demonstrated Conclusion efficacy in relieving organ dysfunctions and shock states, but did not significantly affect the remaining lung parenchyma damage.Copyright © 2022 University of Latvia. All Rights Reserved.

Public health and democratic governance: COVID-19's impact on South Africa's democracy: ZfVP

The research is motivated by the need to determine the impact of South Africa's COVID-19 regulations on its quality of democracy. It takes into account the interests of individual (liberal) rights in competition with the state's interests of public security. Theoretical assumptions, based on classical democratic theories, which rely on the separation of powers and checks-and-balance principles, were used. The South African government architecture is assessed, especially in the context of accountability and oversight requirements. For this purpose, the relationship between the legislature and executive is most relevant. The South African government decided on a state of disaster to manage the pandemic, but it is contrasted with a state of emergency as the constitutional alternative. Its implementation, especially the institutional framework used for it, is analysed. The role of Parliament during the pandemic is used as an important test of the quality of democracy. The conclusions are that South Africa's democratic principles did not degenerate during the pandemic, as concluded by Freedom House, but the pandemic's major impact was on the quality of democracy. The state of disaster's institutions, for example, were not those prescribed by legislation. Moreover, Parliament's involvement in the state of disaster's decision-making was limited. The 2021 local government election, on the other hand, was judged free and fair and its outcomes have been implemented without any public challenges. The main negative outcome is the public's trust deficit in the ANC government's use and abuse of pandemic regulations.

Selected pharmaceutical analysis in a wastewater treatment plant during COVID-19 infection waves in South Africa

Globally, an extensive range of pharmaceuticals are consumed daily to treat a variety of illnesses and diseases. Since the occurrence of the SARS-CoV-2 virus (COVID-19) outbreak, the use of pharmaceuticals has increased drastically in order to treat and prevent infection. Studies have shown that pharmaceutical usage is largely dependent on seasonal temperatures. This was explored in the present study and was verified by the results obtained. Versatile solid phase extraction (SPE) and liquid chromatography-mass spectrometry (LC-MS) methods were developed and validated for the accurate detection of target pharmaceuticals. Method percentage recoveries ranged from 73.53-100.70%, while the limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.0330-0.886 mg L−1 and 0.0990-2.68 mg L−1, respectively. Resulting concentrations of pharmaceuticals used to treat chronic ailments such as diabetes, hypertension, tuberculosis and HIV/AIDS showed consistent daily usage while pharmaceuticals used for the treatment of COVID-19 and influenza showed distinct seasonal trends. Concentrations obtained for sulfamethoxazole hydroxylamine and sulfamethoxazole ranged from 0.05215-0.3438 mg L−1 and 0.009818-0.3002 mg L−1, respectively, while concentrations quantified for prednisolone and ivermectin ranged from 0.008775-0.4482 mg L−1 and 0.008520-0.979 mg L−1, respectively. Trends also directly correlated with the total number of active COVID-19 cases experienced in South Africa during sampling periods and this was confirmed using a one-way ANOVA test. P-values obtained for sulfamethoxazole hydroxylamine, sulfamethoxazole and ivermectin were below 0.05. © 2023 The Royal Society of Chemistry.